N-[[4-(1,4,8,11-Tetraazacyclotetradec-1-ylmethyl)phenyl]methyl]-2-pyridinemethanamine hexahydrobromide
AMD 3465
CAS: 185991-07-5
Molecular Formula: C24H38N6
N-[[4-(1,4,8,11-Tetraazacyclotetradec-1-ylmethyl)phenyl]methyl]-2-pyridinemethanamine hexahydrobromide - Names and Identifiers
N-[[4-(1,4,8,11-Tetraazacyclotetradec-1-ylmethyl)phenyl]methyl]-2-pyridinemethanamine hexahydrobromide - Physico-chemical Properties
| Molecular Formula | C24H38N6 |
| Molar Mass | 410.59872 |
| Melting Point | >200°C (dec.) |
| Solubility | DMSO (Slightly), Methanol (Very Slightly), Water (Slightly) |
| Appearance | powder |
| Color | white to light brown |
| Storage Condition | -20°C |
| In vitro study | AMD3465 blocks the binding of CXCL12 in SupT1 cells with an IC50 of 18 nM. AMD3465 also inhibits cxcl12-induced calcium signaling with an IC50 of 17 nM and inhibits MAPK phosphorylation. However, AMD3465 was unable to block the intracellular calcium flux induced by the CCR5 ligands RANTES, LD78β and MIP-1β in U87.CD4.CCR5 cells. AMD3465 inhibits cxcl12-induced chemotaxis in human T lymphoid SupT1 cells and prevents chemokine-induced CXCR4 internalization in ud7.cd4 cells. In addition, the study showed that AMD3465 can inhibit the activity of X4 HIV-1 strain IIIB, NL4.3, RF and HE, IC50 range 6-12 nM. AMD3465 inhibited ROD and EHO of HIV-2 strains with IC50 of 12.3 nM. AMD3465 inhibited the binding of SDF-1α ligand at K I of 41.7 nM in CCRF-CEM cell line. Inhibition of the CXCR4 signaling pathway by AMD3465 was achieved by inhibiting SDF-1α-stimulated calcium flux and GTP binding with IC50 of 12.07 nM and 10.38 nM, respectively. In addition, AMD3465 can inhibit the physiological response mediated by CXCR4, cell chemotaxis, IC50 is 8.7 nM. In addition, AMD3465 on MIP1α, MCP-1, TARC, RANTES, MIP-3β and IP10 mediated calcium flow, CCR1, The corresponding ligands of CCR2b, CCR4, CCR5, CCR7 and CXCR3, as well as the binding of LTB 4 to BLT1, are not effective, thus indicating that AMD3465 is a selective inhibitor of CXCR4 and not against other chemokines. An independent study showed that AMD3465 could inhibit SDF-1 of CXCR4 internalization mediated in GFP-conjugated CXCR4 expressing cells without receptor internalization when incubated with cells alone. |
| In vivo study | The pharmacokinetics of AMD3465 was studied in mice and dogs. Absorption was rapid after subcutaneous administration. AMD3465 is cleared from dog plasma in biphasic form with a terminal half-life of 1.56-4.63 hours. AMD3465 can be utilized 100% after subcutaneous administration compared to intravenous administration. AMD3465 causes leukopenia after subcutaneous administration in mice and dogs, peaking at 0.5 hours to 1.5 hours after subcutaneous administration in mice, the appearance of the maximum peak plasma concentration in dogs indicates the potential of AMD3465 to mobilize hematopoietic stem cells. AMD3465 terminated type 2 granulation tumor formation and eosinophil mobilization at doses of 6 and 30 mg/kg. Treatment with AMD3465 reduced CXCR4 transcription levels in the lungs with type 2 injury by about 90%. |
N-[[4-(1,4,8,11-Tetraazacyclotetradec-1-ylmethyl)phenyl]methyl]-2-pyridinemethanamine hexahydrobromide - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 1.116 ml | 5.58 ml | 11.16 ml |
| 5 mM | 0.223 ml | 1.116 ml | 2.232 ml |
| 10 mM | 0.112 ml | 0.558 ml | 1.116 ml |
| 5 mM | 0.022 ml | 0.112 ml | 0.223 ml |
Last Update：2024-01-02 23:10:35
N-[[4-(1,4,8,11-Tetraazacyclotetradec-1-ylmethyl)phenyl]methyl]-2-pyridinemethanamine hexahydrobromide - Introduction
AMD 3465 (full name AMD3465 tetrahydrochloride) is a chemical agent that belongs to the CXC chemokine receptor 4(CXCR4) antagonist.
Nature:
AMD 3465 is a white solid with a chemical formula of C19H22Cl2N4O2 and a molecular weight of 415.31g/mol. It has good solubility and can be dissolved in water.
Use:
AMD 3465 is mainly used to study CXCR4 related biological processes and diseases. CXCR4 is a chemokine receptor expressed on the cell membrane, which is involved in the regulation of cell migration, proliferation and survival. The AMD 3465 can be used as a tool to study the CXCR4 pathway in the laboratory for related cell culture experiments and animal experiments.
Preparation Method:
AMD 3465 preparation method is mainly completed by chemical synthesis. Specific synthetic steps need to be performed under laboratory conditions, including the use of appropriate chemical reagents and reaction conditions.
Safety Information:
The use of AMD 3465 requires strict compliance with laboratory safety procedures. Personal protective equipment such as lab gloves and protective glasses should be worn when using AMD 3465. It should avoid contact with skin, eyes and mucous membranes, such as accidental contact, should immediately rinse with plenty of water. When handling and storing AMD 3465, it should be properly managed to avoid mixing with other chemicals, avoiding fire and high temperature. If there are further safety issues, it is recommended to consult and follow the relevant safety information and guidelines.
Nature:
AMD 3465 is a white solid with a chemical formula of C19H22Cl2N4O2 and a molecular weight of 415.31g/mol. It has good solubility and can be dissolved in water.
Use:
AMD 3465 is mainly used to study CXCR4 related biological processes and diseases. CXCR4 is a chemokine receptor expressed on the cell membrane, which is involved in the regulation of cell migration, proliferation and survival. The AMD 3465 can be used as a tool to study the CXCR4 pathway in the laboratory for related cell culture experiments and animal experiments.
Preparation Method:
AMD 3465 preparation method is mainly completed by chemical synthesis. Specific synthetic steps need to be performed under laboratory conditions, including the use of appropriate chemical reagents and reaction conditions.
Safety Information:
The use of AMD 3465 requires strict compliance with laboratory safety procedures. Personal protective equipment such as lab gloves and protective glasses should be worn when using AMD 3465. It should avoid contact with skin, eyes and mucous membranes, such as accidental contact, should immediately rinse with plenty of water. When handling and storing AMD 3465, it should be properly managed to avoid mixing with other chemicals, avoiding fire and high temperature. If there are further safety issues, it is recommended to consult and follow the relevant safety information and guidelines.
Last Update：2024-04-10 22:29:15
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Spot supply
Product Name: AMD 3465 Visit Supplier Webpage Request for quotationCAS: 185991-07-5
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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